We use whole cell patch-clamping combined with neurobiotin injection for the study of pyramidal cell morphology in the rat prefrontal cortex.
The rat prefrontal cortex (PFC) can be considered to be analogous to the human PFC regarding neuronal connections with medial thalamic nuclei and other subcortical areas.
The rat PFC is subdivided into three main subfields: prelimbic, infralimbic,
and anterior cingulate cortex. These subfields seem to have functional
dissimilarities and it has been suggested that they are lateralized.
Until now, however, there are no data concerning morphological
differences in pyramidal cells between these subfields of the right
and the left PFC.
Immunocytochemistry, intracellular neuronal labeling, electrophysiology